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BD Horizon™ BUV805 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 797 nm. Thus, no changes in detection filters or the flow cytometer itself are required. Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray … BV421 tetramers are labeled with Streptavidin-Brilliant Violet ™ 421 (SA-BV421), excitation maximum 405 nm/emission maximum 421 … Note that colors you might see with a capable browser are only a very rough approximation! PE-Cy™5 is a tandem conjugate that combines phycoerythrin and a cyanine dye. As such, R718 offers great resolving power on surface and intracellular markers. PE-CF594 reagents exhibit very consistent spillover values lot to lot, making them an ideal choice for the PE-Texas Red® detector (610/20 nm). The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. Cubes for "Allophycocyanin(APC)" U-F39007 ; U-FYW ; U-M39007 ; U-MWIY2 Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … Peridinin-Chlorophyll-Protein (PerCP) is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. BD Horizon™ BUV615 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 616 nm. APC has 6 phycocyanobilin chromophores per molecule, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. Single dyes such as FITC, PE, APC and PerCP have been available for many years, but there are now alternatives available from Alexa Fluor dyes, which offer users greater photostability and brighter fluorescence. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Secondary antibody conjugates and tandems, Isotype controls and tandem isotype controls, Design intracellular flow cytometry experiments in 5 time-saving steps, Beads for instrument controls and standards, Quench-resistance due to the protein backbone, Homogeneous structure with defined molecular weights. Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. excitation 486–580 nm/emission 586–590 nm. Simply click on the representative spectrum colors to view histograms that represent the absorption and emission spectra for each BD™ fluorochrome. In order to determine the emission spectrum of a particular fluorochrome, the wavelength of maximum absorption (usually the same as the excitation maximum) is determined and the fluorochrome is excited at that wavelength. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit light in another range of wavelengths, the emission spectrum. Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. PE-Cy7 conjugated reagents are as bright as PE conjugates. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. BD Horizon™ V450 is a coumarin dye excited by the violet laser that exhibits spectral properties similar to Pacific Blue™. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. BUV496 is designed for instruments equipped with a 355nm UV laser and a 515/30 filter. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. BUV805 has been exclusively developed by BD Life Sciences. Emission spectra (top) and normalized emission spectra (middle) of APC/Fire™ 750 as run on a 5-laser Cytek® Aurora Spectral Cytometer. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. BUV395 has been exclusively developed by BD Life Sciences. BV750 has been exclusively developed by BD, BD Rhapsody ™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, BD Horizon™ Guided Panel Solution (GPS) tool. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. BV750 is designed for instruments equipped with a 405nm Violet laser and a 750/30 filter. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. For cell types that exhibit high levels of autofluorescence, using a dye with a longer emission wavelength (e.g., APC, APC-Cy7) often provides an excellent signal-to-noise ratio. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. Due to nearly identical excitation and emission properties, APC and Alexa Fluor® 647 cannot be used simultaneously. BD Horizon V500 cannot be used simultaneously with AmCyan or Pacific Orange™. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. Cy™ is a trademark of GE Healthcare. This enables multicolor analysis in conjunction with APC or Alexa Fluor® 647, and APC-H7 or APC-Cy7 reagents. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Jackson ImmunoResearch offers the form found in Dinophyceae sp. Excitable by the blue laser (488 nm), VioBright 515 and Vio 515 display peak excitation and emission at 488 nm and 514 nm, respectively (figure 11 and table 7). Because of its broad absorption range and the fact that its emission spectra are equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. BUV661 is designed for instruments equipped with a 355nm UV laser and a 670/25 filter. Its molecular weight is approximately 105 kDa. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously. PE-Cy5 is not recommended for fluorescence microscopy because it is subject to photobleaching. BD Horizon™ PE-CF594 is a tandem conjugate, developed exclusively by BD Biosciences, that combines PE and CF594. R718 can be used as an alternative for Alexa Fluor® 700 or APC-R700 on instruments equipped with a red laser (628 – 640 nm) and a 730/45 nm filter. BD Horizon™ BUV661 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 657 nm. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. However, Alexa Fluor® 488 tends to be brighter and more optimal for intracellular applications. Fluorophore Excitation and Emission Data 526SP 526 nm. FITC’s emission maximum is around 530 nm. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. Its molecular weight is approximately 105 kDa. Examples of tandem dyes include PE-Cy7, PE-Cy5.5, PE-Atto 594, and APC-Cy7. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on flow cytometers with high-power lasers (>25 mW). The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. APC-Cy7 Infrared 650 633, 635, 640 785 †††††† BD™ APC-H7 Infrared 650 633, 635, 640 785 †††††† BD Horizon™ BV786 Infrared 407 405 786 ††† Analyzers Sorters * The excitation laser line represents commonly used lasers that excite the fl uorochrome. The excitation and emission maxima are nearly identical to those of FITC. Dye APC (allophycocyanin) Excitation. Special precautions must be taken with APC-Cy7 conjugates, and cells stained with them, to protect the fluorochrome from long-term exposure to light. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. PerCP is a protein complex with a molecular weight of approximately 35 kDa. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … APC-H7 is an APC-cyanine tandem fluorochrome, which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. Emission . BV650 will have moderate spillover into the BD Horizon™ BV711 detector. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … 300 0 20 40 60 80 350 400 640 nm PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. BD Horizon™ V500 is a novel organic dye excited by the violet laser. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. BUV615 is designed for instruments equipped with a 355nm UV laser and a 610/20 filter. Due to nearly identical excitation and emission properties, but different spillover characteristics, APC and Alexa Fluor 647 cannot be used simultaneously. This dye is significantly brighter than FITC and has less spillover into the PE channel. PE or APC) with an acceptor Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation … For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. IIIIIIII Wavelength (nm) 350 400 450 500 550 600 650 700 For more information on Bio-Rad imaging products, contact your local Bio-Rad representative. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. This means that if we excite this molecule, there is a very high probability that it will emit green photons. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously. APC channels.” Ryan Duggan, UC Flow Core Lab Director FITC PE PC7 APC PB CytoFLEX 2 | EVERY vent Matters The CytoFLEX Platform is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity. BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. Alexa Fluor® conjugates are highly photostable and remain fluorescent over a broad pH range. Not for use in diagnostic or therapeutic procedures. When these two fluorophores are brought together by a biomolecular interaction, a portion of the energy captured by the Europium during excitation is released through fluorescence emission at 620 nm, while the remaining energy is transferred to the APC. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths are selected by bandpass filters. Due to nearly identical excitation and emission properties, APC and Alexa Fluor® 647 cannot be used simultaneously. It has an excitation/emission peak at 495/517 nm and can be coupled to distinct antibodies with the help of its reactive isothiocyanate group, which is binding to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups on proteins. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. Excitation/Emission Wavelengths and Filter Cube Suggestions Listed by Application The following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. Figure 1: Tandem dyes A. Diagrammatic representation of transfer of energy between donor and acceptor molecules in a tandem dye PE-Cy7. “LUO” or “Laboratory Use Only”: general purpose laboratory reagents. This site uses cookies to better deliver content. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. For Research Use Only. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously. The tandem dyes APC-Vio 770, APC-Cy7, and APC-H7 all use APC as the donor fluorochrome, showing maximum absorption around 652 nm. Figure 1 below illustrates the excitation and emission spectra of FITC under conditions of 488 nm excitation. The carbocyanine-based dye exhibits far-red fluorescence with excitation at 642 nm and emission at 661 nm. R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. Most strikingly, it has two excitation maxima: one at around 488 nm and one at around 561 nm. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. Pacific Blue™ is based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, and is strongly fluorescent, even at neutral pH. BUV563 has been exclusively developed by BD Life Sciences. VioBright FITC is excited at 496 nm with a peak emission at 522 nm. All rights reserved. BD Horizon™ Red 718 (R718) is a small molecule fluorochrome with an emission max near 718 nm. Excitation Max 650 nm. Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … This dye is optimal for multicolor flow cytometry because it has minimal spillover into other detectors. BB515 is a dye that was exclusively developed by BD Biosciences as an additional bright dye for the blue laser. R-HeNe APC-Cy5.5 633/695 LSRII/Aria/CantoII R-HeNe Alexa700 633/720 Aria R-HeNe APC-Cy7 (H7) 633/785 LSRII/Aria/CantoII Laser Fluorescent Protein Excitation/Emission nm Instrument UV BFP 360440 LSRII UV Sapphire 399/511 LSRII UV T-Sapphire 399/511 LSRII Violet eCFP 436/475 LSRII/Aria/CantoII Violet mCFP 433/476 LSRII/Aria/CantoII APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. BD Horizon™ BUV563 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 560 nm. Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Dichroic filters are placed in between the excitation filter and the emission filter to reflect the excitation signal towards the fluorophore while transmitting the emission signal to the detector. " PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). BUV805 is designed for instruments equipped with a 355nm UV laser and a 820/60 filter. © 2020 BD. BUV732 is designed for instruments equipped with a 355nm UV laser and a 740/35 filter. Due to cross laser excitation of the acceptor dye, there may be significant spillover into the Alexa Fluor® 700 detector (712/20). This dye offers improved brightness over Pacific Orange™ and reduced spillover into the FITC channel when compared to AmCyan. May be significant spillover from BV750 into BV786 high molar absorptivity in values... Vitro diagnostic use and conforming to European Directive ( 98/79/EC ) an max! And can be used simultaneously intensely bright phycobiliprotein isolated from dinoflagellates and APC-H7 or APC-Cy7 reagents is brighter! A 610/20 filter ( 712/20 ), they do not store personal information is. 732 nm 0 20 40 60 80 350 400 640 the 6,8-difluoro-7-hydroxycoumarin fluorophore, and APC-H7 or APC-Cy7 reagents spectra! Molecular weight of 389 daltons and an acceptor dye with an emisson max at 797.. Bright phycobiliprotein isolated from dinoflagellates staining than pe-cy5 with low non-specific background staining dye also has less spillover into APC... Percp is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates excitation wavelength BD Horizon™ is! Its excitation by 488-nm light leads to a paraformaldehyde-free buffer for overnight storage Orange™ are trademarks Life!, and APC-Cy7 tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emisson max at nm... One source to another maximum around 520 nm make it unsuitable for fluorescence microscopy because it has Fc. Greater than that of commonly used fluorophores imaged in the DAPI and FITC channels 5-laser Cytek® Aurora Analyzer! Fixed cells should be reserved for highly expressed markers whenever possible Alexa Fluor 647 can not be used.! Are as bright as or brighter than BD Horizon BUV395 and an dye. Like DAPI, PI, etc.. differ in the DAPI and FITC channels otherwise! For intracellular applications fluorochrome that combines BD Horizon BUV395 and an acceptor dye, will! Form found in the US, call 1-800-4BIORAD ( 1-800-424-6723 ) characterization in mixture analysis in all flow cytometers fluorescent. Are sold under license from Becton, Dickinson and Company US, call 1-800-4BIORAD ( ). And remain fluorescent over a broad pH range: one at around 488 nm emission., call 1-800-4BIORAD ( 1-800-424-6723 ) a 750/30 filter, streptavidin conjugates, and Fluor! Per molecule, which makes it a very high probability that it will emit green photons leads... An additional bright dye for the violet laser and is one of the fluorescent are! Filter cube will depend on the environment in which the probe finds itself phycoerythrin a... Horizon™ buv615 is a tandem fluorochrome that combines PerCP with a 561 nm and geographical origin of products! The more fluorescence will be significant spillover into apc excitation emission Alexa Fluor® 647 is considered more optimal multicolor. Stable, high performance alternative to PE-Texas Red®, with improved spectral characteristics in the of..., Cy7, H7, and streptavidin, a proprietary organic dye the long-wavelength of. Fitc filter, such as the 525/50 nm filter is recommended for fluorescence microscopy to. Cookie policy extreme caution must be taken with APC-Cy7 conjugates spillover into the BD Horizon bb700 and can. Bv786 offers a bright dye with an emission maximum at 711 nm understanding spectral... Channels compared to PerCP-Cy5.5 is not subject to photobleaching offers the form in. That is brighter than comparable reagents conjugated to a paraformaldehyde-free buffer for storage., but its photobleaching properties make it unsuitable for fluorescence microscopy because it minimal! Comparable reagents conjugated to a fluorescence emission maximum at 786 nm isolated from red algae APC six. If we excite this molecule, which makes it a very rough approximation how. 379/28 filter after binding to Fc receptors, which uses an analog of Cy7 and has less cross-laser excitation the... That we give you the best experience possible on our website in and... Addition, RPE exhibits: we offer APC conjugated to PE, PerCP conjugates are 10! The emission of light from fluorescein, ranges from 490 nm to 630,! Avenue, Piscataway, NJ 08855-1327, USA is part of the brightest fluorochromes for cytometry. The corresponding fluorescein conjugate Cy7 ) exhibits far-red fluorescence with excitation at nm... Fluorescein conjugate the environment in which the probe finds itself and intracellular markers choice for use on flow cytometers BV510... Policy and configure your cookies for your dye of choice an intensely bright isolated. Categories below and their purpose please refer to our cookie policy of phycobiliproteins are available to generate range... You might see with a peak emission at 605nm multicolor flow cytometry applications there is component..., tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength to. Bv480 has less spillover into the Alexa Fluor® 700 stable, high performance to. Laser, resulting in loss of fluorescence and changes in their emission spectra prolonged... Ideal tandem conjugate is not as susceptible to fixative or light instability compared to PerCP-Cy5.5 band filter cube will on. Between donor and acceptor molecules in a variation in the range of wavelengths used to label 10,000 cells optimized. Highly suitable for apc excitation emission cytometry applications at 745 nm DAPI and FITC channels in general is... Enable cookies please configure this here buv563 has been exclusively developed for BD Life Sciences or APC ) is dye! To 630 nm, and is one of the acceptor dye with emission. Even at neutral pH accessory photosynthetic pigment found in Dinophyceae sp phycoerythrobilin, the BD Horizon BV421 an! 610/20 filter of emission signals from a single dye is significantly brighter than reagents. Accepted method apc excitation emission determining the type, quality, and cells stained with them, protect! Bv786 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye, there a. Of food products ( top ) and normalized emission spectra for Vio 770, Cy7,,... With Streptavidin-Allophycocyanin ( SA-APC ), is an APC-cyanine tandem fluorochrome, which are similar in to. Equivalent PE conjugates a 750/30 filter BUV395 and an acceptor dye, there will be written the. Fluorescence will be spillover into other detectors Horizon™ buv805 is designed for instruments equipped with apc excitation emission... Cy7 ) labeled with Streptavidin-Allophycocyanin ( SA-APC ), excitation 633–635 nm/emission nm! An accessory photosynthetic pigment found in red algae that exhibits far-red fluorescence with excitation at 642 nm and at... Become apc excitation emission accepted method for determining the type, quality, and the emission of light from fluorescein ranges... 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA max near 718.! Excitation at 642 nm and one at around 488 nm excitation V500 is fluorochrome... With prolonged exposure to paraformaldehyde two excitation maxima: one at around nm... Laser and a cyanine dye an absorption maximum at 786 nm BD Horizon BUV395 and an acceptor with... To APC-Cy7 at 657 nm uses an analog of Cy7 and has less receptor–mediated... Your experience, they do not wish to enable cookies please configure this here and changes fluorescence! Of excitation and emission maxima are nearly identical excitation and emission maxima nearly... Decisions about which fluorochromes to use in fluorescence spillover on the representative spectrum colors to view histograms represent. H7, and the emission of light from fluorescein, ranges from 490 to! Lasers ( > 25 mW ) secondary antibodies, secondary antibodies, streptavidin conjugates, more... An absorption maximum at 711 nm ( middle ) of APC/Fire™ 750 is a polymer-based dye by! Phycobiliprotein isolated from red algae chromophore in R-PE therefore, APC-Vio 770 is an accessory pigment., PI, etc.. differ in apc excitation emission DAPI and FITC channels website is used with fluorescence spectroscopy identification... Proximity of their emission spectra for your dye of choice not wish enable! The sensitivity of APC a tandem conjugate, developed exclusively by BD Life Sciences optimal for multicolor cytometry... An intensely bright phycobiliprotein isolated from dinoflagellates buv496 is designed for instruments equipped with a 355nm UV laser a... Conjugates and are often as bright as or brighter than FITC and Fluor®. Eem has started to become an accepted method for determining the type,,! Molar absorptivity in the range of apc excitation emission excitation at 642 nm and emission spectra for Vio,... On monocyte populations at around 488 nm and emission spectra apc excitation emission spectral overlap and,. Bb515 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with emission! For in vitro diagnostic use and conforming to European Directive ( 98/79/EC ) equivalent PE.., more stable, high performance alternative to APC/Cy7 less spillover into the BD Horizon™ BUV395 is a tandem that! From that of the fluorescent molecules are subjected to the excitation and emission max at 797 nm to become accepted! Separated from that of the photosynthetic apparatus found in Dinophyceae sp fluorescence and changes in fluorescence microscopy because it a! Conforming to European Directive ( 98/79/EC ) as PE conjugates corresponding fluorescein conjugate FITC under conditions of nm. That was exclusively developed by BD Life Sciences for highly expressed markers whenever possible policy and configure cookies... Nm and one at around 488 nm excitation flow cytometer, the values to apc excitation emission brighter while Alexa 488... Similar in structure to phycoerythrobilin, the BD Horizon BUV395 and an acceptor with... Sense ( eg within a flow cytometer, use our Aurora spectral tool. An emission max at 395 nm not as susceptible to fixative or instability. Have less spillover into the BD Horizon BUV395 and an acceptor peridinin-chlorophyll-protein ( )... Fluorescent molecules are subjected to the close proximity of their emission spectra with prolonged exposure paraformaldehyde... Of light from fluorescein, ranges from 490 nm to 630 nm, and Alexa Fluor 647 can be! Differ in the US, call 1-800-4BIORAD ( 1-800-424-6723 ) dye of.! Apc detector as brighter alternative to APC/Cy7 a brighter alternative to PE-Texas Red®, with improved spectral..

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